1:BioMedical Genomics Center: Advancing genomics research, education, & outreach° From
its inception in the spring of 2000, the BioMedical Genomics Center at
the University of Minnesota has been advancing genomics and proteomics
research as it applies to human and animal health. The BMGC continues
to maintain, upgrade, and acquire state-of-the-art instrumentation for
genomics and proteomics-focused research.The BMGC is a leader in the
development of genomics-related education and outreach through
specialized conferences, seminars, and workshops. Through these
efforts, the BMGC has helped establish entrepreneurial pathways linking
genomics and proteomics discoveries to the founding of knowledge-based
companies in the state of Minnesota. Staff DirectoryKenneth Beckman, Ph.D., Director kbeckman@umn.edu 612.626.2985 MICROARRAY FACILITY - 612.624.0437 affy@umn.edu· Aaron Becker, Assistant Scientist
becke044@umn.edu· Archana Deshpande, Scientist
deshp008@umn.edu GENOTYPING / OLIGONUCLEOTIDE & PEPTIDE SYNTHESIS FACILITY - 612.624.3177 oligo@umn.edu· Dinesha Walek, Scientist
walek001@umn.edu· Ann Bohac, Scientist
bohac001@umn.edu DNA SEQUENCING AND ANALYSIS FACILITY - 612.625.7736 bmgcseq@umn.edu - Sanger Sequencinggsflx@umn.edu - Next Generation Sequencing· Kari Bunjer, Scientist
bunje001@umn.edu· Nichole Peterson, Scientist
peter585@umn.edu· Jiangming Deng, Assistant Scientist
dengx009@umn.edu· Xia Li, Assistant Scientist
lixxx081@umn.edu RNAi CORE FACILITY - 612.626.5104 rnai@umn.edu· Dr. Wade Bresnahan, Program Director
bresn013@umn.edu· Adam Hauge, Junior Scientist
haug0296@umn.edu 2: Biotechnology Resource Center For researchers at the University and in industry, the Biotechnology Resource Center offers customized research & development services as well as limited production services in the following areas:  Fermentation
Down Stream Processing Molecular Biology Protein Expression At
the Biotechnology Resource Center we have experience with fermentations
using a wide variety of bacteria, yeast, and fungi. The services we
offer include: strain development media development and optimization overall process development scale-up biomass production primary and secondary metabolite production The
Biotechnology Resource center offers capabilities for the recovery and
purification of cells, proteins, as well as primary and secondary
metabolites from the bench-scale to the pilot-scale. Services include: production of frozen cell pellets cell disruption inclusion body preparations product harvest and concentration by micro- and ultrafiltration chromatographic purification (ion-exchange, affinity, size exclusion) protein refolding freeze drying Our team of molecular biologists has extensive experience with bacterial, yeast, animal, and plant genetics. Services include: cloning of genes site-directed and random mutagenesis sequence verification construction of expression vectors Our work in protein expression integrates most of the services described above. Our primary expression hosts are E. coli, Pichia pastoris, and the Baculovirus/insect
cell system. However, other organisms can be used as well. A complete
protein expression project would comprise the following steps: cloning target gene into chosen expression vectorµµ creation of fusion proteins, if desired incorporating expression vector into expression host initial protein expression analysis on a small scale expression optimization specific to host organism scale-up of fermentation or cell culture to desired process size development and optimization of protein recovery and purification strategy scale-up of recovery and purification process DIRECTOR / Marc von Keitz, Ph.D. Telephone: (612) 624-6758 E-mail: vonkeitz@umn.edu RESEARCH ASSOCIATE / Fred Schendel, Ph.D.
Telephone: (612) 624-5347
E-mail: schen013@umn.edu | 
